MxT = Microfluidics x Therapy

Microfluidic Gene Editing for Cell Therapy Starts Right Here

We transform cancer immunotherapy, cellular engineering and genome editing with our patented non-viral genome editing technology, Hydroporator .

TECHNOLOGY

Hydroporator : Next-Generation Microfluidic cellular engineering platform accelerating the discovery, development and manufacturing of novel cell therapies

Hydroporator is a non-viral microfluidic platform for highly effective delivery of external cargos such as plasmid DNA, mRNA, proteins, CRISPR-Cas9 RNPs, and nanoparticles into primary cells. The Hydroporator technology enables high delivery eﬃciency, easy operation, low cost, high scalability, minimal cell perturbation (i.e., preserved cell function and viability), and cell type/cargo insensitive delivery. Hydroporator
is free from the challenges from viral transduction, lipofection and electroporation, establishing a new avenue to accelerate cancer immunotherapy, cellular engineering and genome editing.

Publication

Fundamental advantages

Unlimited in cargo type including large sizes (>10kB)

Maintains viability (%90)

Highly efficient (e.g. >80 mRNA)

Scalable from R&D into clinic

Effective for variety of cells (including adherent)

Preserved Cell Viability & Function

High Speed & Throughput Capability

Simple and Scalable

Strong Clinical Applicability

Technologies	Efficiency	Nanoparticle delivery (> 100 nm)	Primary cell applicability	Viability	Scalability (per run)	Cost
Electroporation	(depends on cell and cargo type)	(high Stokes drag)	(low viability)	(depends on cell type)	High(104-106 cells per run)	$10K to 100K
Microinjection	(Theoretically high)			(depends on cell type)	Low(100 cells per hour)	$10K(Injector only)
Viral transduction	(Limited in DNA size)	(packaging failure)		(Mutagenesis concern)	Low to High(depends on viral amount)	High(preparation)
Lipofection	(depends on cell and cargo type)	(packaging failure)	(Low efficiency for suspension cells)	(depends on cell type)	Low to High(depends on reagent amount)	$1K/50 tests
Hydroporator™	(>90% for human primary T cell)	(> 300 nm)	(Immunocyte, stem cell....)	(> 80%)	High(106 cells per min per channel)	~$1(Chip fabrication)